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为了解已选育的小麦品系中抗白粉病基因和优质基因分布和聚合的情况,利用Pm4的STS标记,Pm21的SCAR标记,以及Dx5的特异扩增序列对F5、F6、F8和F9代的146份小麦中、高代品系进行了检测.检测表明,4个世代中,有9份品系检测到含有Pm4基因,检出率为6.16%;2份品系检测到含有Pm21基因,检出率为1.37%;有140份品系检测到含有Dx5基因,检出率为95.9%.在检测的所有品系中,同时聚合了Pm4和Dx5基因的材料有9份,分别是K5-19-1、K8-71-1、K8-72、K8-73-1、K8-79、K8-80、K8-81、K8-82和K9-5;同时聚合了Pm21和Dx5基因的材料有2份,分别是K8-31-1和K8-55.没有检测到3个基因同时聚合的情况.“,”To understand the distribution and polymerization of powdery mildew resistance genes and high quality genes in selected breeding wheat lines,the STS markers of Pm4,SCAR markers of Pm21,and specific sequence amplification of Dx5 were used to detect 146 middle and high generation breeding lines of F5,F6,F8 and F9.The results showed that in the four generations,9 breeding lines were detected carrying Pm4 gene,and the detection rate was 6.16%.2 breeding lines were detected carrying Pm21 gene,and the detection rate was 1.37%.140 breeding lines were detected carrying Dx5 gene,and the detection rate was 95.9%.In all the breeding lines detected,9 breeding lines simultaneously polymerized Pm4 and Dx5 genes.They were K5-19-1,K8-71-1,K8-72,K8-73-1,K8-79,K8-80,K8-81,K8-82 and K9-5.2 breeding lines simultaneously polymerized Pm21 and Dx5 genes.They were K8-31-1 and K8-55.Simultaneous polymerization of all the three genes were not found.