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以马大杂种相思为研究对象,建立扦插基质简单、成分可控,不需特殊培养条件的瓶内扦插生根技术体系。为今后深入研究扦插生根机理,提供一种扦插基质成分及培养环境可控的技术平台,利于获得更准确、可靠的生根机理研究结果。8月采取马大杂种相思采穗圃的当年生腋芽饱满枝条为外植体,扦插基质由7 g/L琼脂和抑菌剂(多菌灵,百菌清)组成,筛选适宜的抑菌剂种类及浓度,结合对外植体类型和消毒方式的筛选,确定适宜的外植体类型及消毒方式;对IBA、NAA、ABT三种生长调节剂的浓度和处理时间进行研究,最终确定适宜的扦插生根处理。结果表明:两种抑菌剂的抑菌效果有显著差异,以百菌清为适宜的抑菌剂,浓度为0.2 g/L最佳;以中部带叶为最佳外植体,并以多菌灵0.8 g/L浸泡3 min对外植体进行预处理,并IBA 1.0 g/L处理2 h,在开放的实验台上接种至扦插基质中,生根率为100%。本研究在扦插基质中加入抑菌剂,不使用高温高压灭菌、超净工作台,建立了马大杂种相思开放式瓶内生根体系,对后续生根机理研究具有重要意义。
In this study, hybrids of Acacia as the research object were set up to establish the rooting technology system of cuttings in bottle with simple matrix composition, controllable composition and no special culture conditions. For the future in-depth study cutting rooting mechanism to provide a cutting matrix composition and culture environment controllable technology platform conducive to obtaining more accurate and reliable results of the rooting mechanism. August to take the horse hybrid Acacia sprouts in the annual axillary buds full of explants, cutting substrate by the 7 g / L agar and antibacterial agents (carbendazim, chlorothalonil) composition, screening of suitable antibacterial agents The types and concentrations of IBA, NAA and ABT, and the screening of explant types and disinfection methods to determine the appropriate explant types and disinfection methods; the concentration of IBA, NAA, ABT growth regulators and treatment time were studied to determine the appropriate cuttings Root treatment. The results showed that the antibacterial effect of the two antibacterials were significantly different. Chlorothalonil was the best bacteriostatic agent with the best concentration of 0.2 g / L. The explants were pretreated with 0.8 g / L mycelium for 3 min and treated with IBA at 1.0 g / L for 2 h. The explants were inoculated into the cutting medium on an open experimental bench and the rooting rate was 100%. In this study, the addition of bacteriostatic agents to the cutting medium without the use of autoclave and ultra clean bench to establish open-topped rooting system of Acacia hybrids is of great significance to the study of subsequent rooting mechanism.