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A simple method has been developed to prepare cuprous selenide nanocrystals by the reaction of copper nitrate trihydrate with selenium and sodium mercaptoacetate in aqueous ammonia system. Cu2Se nanocrystals were characterized by transmission electron microscopy (TEM), high resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), electron diffraction (ED), fluorescence spectrum and ultraviolet-visible absorption spectrum. Cu2Se nanocrystals showed berzelianite structure with 20-40 nm in length and 10-20 nm in width. A possible mechanism is also discussed.