论文部分内容阅读
在田间进行2组试验以分析甘蔗分离群体的甘蔗黑穗病病情指数。新植蔗和宿根蔗分别有72份和50份无性系参试。采用孢子悬浮液浸渍法对新植蔗进行接种,并收集新植蔗和宿根蔗自第1个黑穗病鞭子出现至发病停止期间的数据。对所收集到的新植蔗3个病情指数茎感染率(SI)、丛感染率(PI)和病害进展曲线下的面积(AUDPC)的数据进行正态分布检验。结果表明:AUDPC与标准正态分布曲线最吻合,其3个特征值正态度、偏度和峰度分别为0.949,0.018和-0.721;PI的吻合程度次之;SI为第3。以上结果的置信度大于97.7%。不论新植蔗还是宿根蔗,3个病情指数两两间以及各病情指数与持续发病期(SDD)间均呈显著正相关,在AUDPC与SI间的相关系数最大,新、宿分别为0.929和0.888。除潜伏侵染期(LP)与PI间的相关外,LP与AUDPC、SI和SDD间的负相关均达到显著水平,LP与SDD间的负相关系数最大,为0.521。相关分析结果暗示,在进行甘蔗抗黑穗病性评价时,利用包括病情指数和病害流行学参数在内的多个参数综合评价抗病性是必要的。
Two sets of experiments were conducted in the field to analyze the disease index of sugarcane smut in sugarcane-separated populations. There were 72 new sugarcane and ratoon sugarcane and 50 clones respectively. The new sugarcane was inoculated by spore suspension immersion method and the data of new plant sugarcane and sugarcane were collected from the first smut to the onset of disease. The results of the normal distribution test of the three disease index (SI), the plexus infection rate (PI) and the area under the disease progression curve (AUDPC) of the three disease indices collected from the new sugarcane plants were tested. The results show that AUDPC is in good agreement with the standard normal distribution curve, and the three eigenvalues of positive, skewness and kurtosis are 0.949, 0.018 and -0.721, respectively; The above result is more than 97.7% confidence level. There was a significant positive correlation between the three disease index and the disease index and the duration of onset (SDD) of newly planted sugarcane or dormant sugarcane. The correlation coefficient between AUDPC and SI was the largest, with the highest of 0.929 0.888. In addition to the correlation between latent infection period (LP) and PI, the negative correlation between LP and AUDPC, SI and SDD reached significant level, and the negative correlation coefficient between LP and SDD was the highest (0.521). Correlation analysis indicated that it is necessary to comprehensively evaluate the disease resistance by using multiple parameters including disease index and epidemiological parameters of disease resistance in sugarcane resistance evaluation.