苹果新梢的无菌培养,报道最早的是 Elliott(1972)and Walkey(1972)。随之,很多品种培养成功,并发展了离体培养繁殖程序。高的新梢增殖速度和生根频率对大规模繁殖和获得更多砧木和接穗品种是有益的(Jones 等1977,1979;Lane 1978)。使用的培养基则因报道的实验室和培养的品种而不同。在以往的所有报道中,我们尚未发现为了新梢增殖(细胞激动素)和生根启动(生长素),对不同品种苹果新梢培养体所必需的生长调节剂的浓度范围比较 Aseptic cultivation of apple shoots was first reported by Elliott (1972) and Walkey (1972). As a result, many varieties have been successfully cultivated and have developed in vitro culture and reproduction programs. High shoot propagation rate and rooting frequency are beneficial for large-scale reproduction and access to more rootstocks and scion varieties (Jones et al. 1977, 1979; Lane 1978). The medium used will vary depending on the laboratory being tested and the type of culture. In all of the previous reports, we have not found a comparison of the concentration range of growth regulators necessary for shoot induction of different varieties of apple shoots for shoot proliferation (auxin) and rooting (auxin)