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目的:应用蛋白组学技术对肾错构瘤尿液进行差异蛋白研究,以提高肾错构瘤的诊断率。方法:应用磁珠联合MALDI-TOF-MS技术对肾错构瘤尿液进行差异蛋白研究,筛选出肾错构瘤多个尿液差异蛋白,并应用遗传算法建立诊断模型。结果:筛选出肾错构瘤与对照组之间差异蛋白峰124个,具有较明显差异的蛋白峰41个(P<0.05)。利用ClinProTools 2.2软件通过遗传算法优化选择,筛选出符合条件的12个差异蛋白峰建立诊断模型,交叉验证识别能力为91.67%。结论:应用蛋白组学技术对肾错构瘤尿液进行差异蛋白研究具有很好的重复性、可行性和可信性。首次应用遗传算法建立肾错构瘤尿液差异蛋白的诊断模型,该模型识别能力高,有助于提高肾错构瘤的诊断率。
OBJECTIVE: To study the differentially expressed proteins of renal hamartoma by proteomic techniques to improve the diagnostic rate of renal hamartoma. Methods: The differential proteins of urine of renal hamartoma were studied by magnetic beads combined with MALDI-TOF-MS. Several urine proteins of renal hamartoma were screened out, and a diagnostic model was established by genetic algorithm. Results: There were 124 differentially expressed protein peaks between the renal hamartoma and control group, 41 of which had obvious difference (P <0.05). ClinProTools 2.2 software was used to optimize the selection by genetic algorithm, 12 qualified protein peaks were screened out to establish a diagnostic model, and the cross validation ability was 91.67%. Conclusion: The proteomics technique has good repeatability, feasibility and credibility for differential protein analysis of renal hamartoma urine. For the first time, a genetic algorithm was used to establish a diagnostic model of urine differential proteins in renal hamartoma. This model has high recognition ability and helps to improve the diagnostic rate of renal hamartoma.