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[目的]探讨苦杏仁苷促进人血T淋巴细胞早熟凝集染色体(PCC)分裂增殖的作用,为辐射损伤快速生物剂量估算提供依据。[方法]每一实验项目取6名不同实验者的周围血加入不同浓度的苦杏仁苷进行实验。姐妹染色单体互换(SCE)方法用于观察苦杏仁苷最佳增殖浓度和促进细胞进入下一分裂周期的作用,以第2代细胞率为指标;常规的PCC方法(培养48h)和本研究的PCC方法(培养40h、加不同浓度苦杏仁苷)用于观察PCC细胞的G1、S、G2和M期,以G2+M期PCC细胞率作评价指标。[结果]①每一实验者苦杏仁苷均明显促进T淋巴细胞的增殖(P<0.01～P<0.005),最佳浓度为每L培养体系含200mg苦杏仁苷,比0mg/L的第2代细胞率增加0.74～1.60倍。②以苦杏仁苷促进细胞增殖的最佳浓度培养体系,培养28h加入秋水仙素的时间点出现第2代细胞(5‰～9‰);而未加苦杏仁苷的样本要在培养36h加秋水仙才出现第2代细胞(4‰～9‰)提早8h。③以苦杏仁苷最佳浓度配合花萼海绵体诱癌素A的培养体系培养,获得T淋巴细胞G2+M期的PCC细胞率最高(68.5%±31.0%);与0mg/L浓度组(26.8%±15.7%)及常规方法(38.17%±18.56%)比较,差异均有统计学意义(P<0.01和P<0.05)。[结论]苦杏仁苷有明显促进人血T淋巴细胞PCC增殖的作用;可缩短细胞分裂周期;对于辐射损伤应急生物剂量估算有重要的应用价值。
[Objective] To explore the effect of amygdalin on the proliferation and proliferation of precocious aggregated chromosomes (PCC) of human T lymphocytes and provide the basis for rapid biological dose estimation of radiation injury. [Method] Peripheral blood from 6 different experimenters was added into experiments with different concentrations of amygdalin. Sister chromatid exchange (SCE) method was used to observe the best proliferative concentration of amygdalin and promote cell entry into the next division cycle, with the second generation of cell rate as an indicator; conventional PCC method (48h) The study of PCC method (cultured 40h, with different concentrations of amygdalin) for the observation of PCC cell G1, S, G2 and M phase, G2 + M PCC cell rate as an index. [Results] ① Adenosine of each experimenter significantly promoted the proliferation of T lymphocytes (P <0.01 ~ P <0.005), and the optimal concentration was 200 mg of amygdalin per L culture system, more than that of 0 mg / L of 2 Generation of cells increased by 0.74 to 1.60 times. ② the best concentration of amygdalin to promote cell proliferation culture system, the second generation of cells (5 ‰ ~ 9 ‰) appeared at 28h after the addition of colchicine, while the sample without added amygdalin was cultured for 36h Autumn Narcissus appeared second generation cells (4 ‰ ~ 9 ‰) 8h earlier. ③ The highest PCC cell rate (68.5% ± 31.0%) in G2 + M phase of T lymphocytes was obtained with the best concentration of amygdalin combined with the culture of Calyx cavernosum. % ± 15.7%) and routine methods (38.17% ± 18.56%), the differences were statistically significant (P <0.01 and P <0.05). [Conclusion] Amygdalin can significantly promote the proliferation of PCC in human T lymphocytes and shorten the period of cell division. It is of great value in the evaluation of emergency biological dose for radiation injury.