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目的观察骨碱性磷酸酶(BALP)及Ⅰ型胶原羧基端前肽(CⅠCP)在燃煤型氟中毒大鼠中的表达变化,探讨骨形成在氟中毒骨病变中的地位。方法将断乳4周的SD大鼠160只随机分为对照组、低氟组、中氟组、高氟组(饲料氟:6.30、9.56、15.89、23.00 mg/kg),食用病区煤烘玉米,复制燃煤型氟中毒动物模型。分别于染氟后30、90、180 d以ELISA法检测血清BALP和CⅠCP水平。结果染氟30和90 d时各组动物血清BALP水平差异无统计学意义,180 d时中氟组、高氟组BALP均高于对照组和低氟组(P<0.01)。染氟30 d时高氟组血清CⅠCP水平低于对照组(P<0.05);90 d时中氟组和高氟组血清CⅠCP水平均高于对照组(P<0.05),且有随着剂量增加而增加的趋势;180 d时各组动物血清CⅠCP水平高于对照组(P<0.01),且有随着剂量增加而增加的趋势。结论氟可以使大鼠骨形成加速,且氟对CⅠCP表达的影响较BALP显著。
Objective To observe the expression changes of bone alkaline phosphatase (BALP) and type Ⅰ collagen carboxyterminal propeptide (C ⅠCP) in coal-exposed fluorosis rats and explore the role of bone formation in fluorosis-induced bone lesions. Methods 160 SD rats were randomly divided into control group, low fluoride group, middle fluoride group and high fluoride group (feed fluoride: 6.30,9.56,15.89,23.00 mg / kg) Corn, duplicate coal-fired fluorosis animal model. Serum BALP and CⅠCP levels were detected by ELISA at 30, 90 and 180 days after fluoride exposure. Results There was no significant difference in serum BALP levels between the two groups at 30 d and 90 d. At 180 d, BALP in middle and high fluoride groups were significantly higher than those in control group and low fluoride group (P <0.01). Serum CⅠCP levels in high fluoride group were lower than those in control group at 30 d after fluoridation (P <0.05); serum CⅠCP level in middle fluoride group and high fluoride group were higher than that of control group at 90 d (P <0.05) (P <0.01). The levels of serum CⅠCP in each group were higher than those in the control group at 180 d (P <0.01), and increased with the increase of the dosage. Conclusion Fluoride can accelerate the formation of bone in rats, and the effect of fluoride on C Ⅰ CP expression is more significant than BALP.