【摘 要】
：
本文构建DNA聚合酶I的新型DNA电化学传感器,将捕获探针通过Au—S键固定于Au基底表面,与互补靶序列杂交至点突变前一个碱基,通过DNA聚合酶Ⅰ将d UTP-biotin连接在目标DNA的检
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本文构建DNA聚合酶I的新型DNA电化学传感器,将捕获探针通过Au—S键固定于Au基底表面,与互补靶序列杂交至点突变前一个碱基,通过DNA聚合酶Ⅰ将d UTP-biotin连接在目标DNA的检测位点,再与avidin-HRP反应,而后测定在TMB溶液中的电化学特性.结果表明,DNA电化学传感电极的检测电流值与K-ras突变型基因浓度(1.0×10-15~1.0×10-10mol·L-1)对数呈良好的线性关系,且灵敏度高,特异性较佳.
In this paper, a new DNA electrochemical sensor of DNA polymerase I was constructed. The capture probe was immobilized on Au substrate by Au-S bond and hybridized with complementary target sequence to one base before point mutation. DNA polymerase I was used to transfer d UTP- biotin was connected to the detection site of the target DNA, and then reacted with avidin-HRP, and then the electrochemical characteristics in TMB solution were determined.The results showed that the detection current value of DNA electrochemical sensing electrode and K-ras mutant gene concentration 1.0 × 10-15 ~ 1.0 × 10-10mol·L-1) logarithm showed a good linear relationship, and high sensitivity, better specificity.
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