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为了明确引起云南省昆明市杨林镇烟草漂浮育苗过程中出现的根腐病的病原,在该烟草漂浮育苗基地取3点,分别在各点采集病株10个样本,烟草品种为NC297,对样本进行了病原菌分离、致病性测定、形态学鉴定和5.8 rDNA-ITS序列分析。结果表明,致病性试验中烟苗发病率为100%;病原菌的5.8 rDNA-ITS序列与GenBank:AY666087.1(Pythium diclinum Tokunaga in Ito&Tokunaga)同源性为99%;异丝腐霉通过黄瓜片诱导后能产生大量孢子;引起该烟草漂浮育苗根腐病的病原菌为异丝腐霉(Pythium diclinum);根据症状和病原菌,将此病害定为由异丝腐霉(Pythium diclinum)引起的烟草漂浮育苗根腐病;异丝腐霉引起烟草根腐病为首次报道。
In order to clarify the pathogen of root rot appeared during the process of tobacco floating seedling in Yanglin Town, Kunming, Yunnan Province, three samples of the diseased tobacco seedling were collected at each point. The tobacco variety NC297, Samples were isolated from pathogens, pathogenicity, morphological identification and 5.8 rDNA-ITS sequence analysis. The results showed that the incidence of tobacco seedling was 100% in pathogenicity test. The 5.8 rDNA-ITS sequence of pathogen was 99% homologous to GenBank: AY666087.1 (Pythium diclinum Tokunaga in Ito & Tokunaga) After the induction, a large number of spores were produced; the pathogen causing the root rot of the nursery floating seedlings was Pythium diclinum; the disease was identified as tobacco floating caused by Pythium diclinum according to the symptoms and pathogenic bacteria Nursery root rot; Tobacco root rot caused by Phlox is the first report.