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目的观察补骨脂、双氢青蒿素及二者配伍治疗隐孢子虫感染小鼠前后的肠壁黏膜组织病理变化和T细胞表面的CD28分子表达水平,探讨这3组中药对隐孢子虫病防治的疗效。方法 60只昆明种小鼠随机分成正常组A、阳性对照组B、补骨脂治疗组C、双氢青蒿素治疗组D以及二者配伍治疗组E。经1和2周药物治疗后,观察各组小鼠肠壁黏膜组织病理变化和T细胞表面的CD28分子表达水平。结果经过1和2周的治疗,药物治疗组小鼠的粪便卵囊量与阳性对照组比较,均明显减少(P<0.05);小肠病理学显示,小肠上皮处于修复之中。在经药物治疗后,所有治疗组的CD28分子表达明显高于阳性对照组B1(P<0.05),补骨脂治疗组C2最高(64.01±5.39),明显高于双氢青蒿素治疗组D2(P<0.05)。结论补骨脂和双氢青蒿素能抑杀隐孢子虫,其中双氢青蒿素杀虫效果明显,而补骨脂主要通过调节外周血CD28分子,以及CD4+与CD3+T细胞比例,二者通过协同作用,参与宿主免疫应答和炎症反应过程,最终修复炎症反应。
Objective To observe the pathological changes of intestinal mucosa and the expression of CD28 on the surface of T lymphocytes in mice treated with psoralen, dihydroartemisinin and their combination before and after treatment with Cryptosporidium, and to explore the effect of these three groups on Cryptosporidiosis Prevention and treatment of the effect. Methods Sixty Kunming mice were randomly divided into normal group A, positive control group B, psoralen treatment group C, dihydroartemisinin treatment group D and compatibility treatment group E. After 1 and 2 weeks of drug treatment, the pathological changes of intestinal mucosa and the expression of CD28 on T cells were observed. Results After 1 and 2 weeks of treatment, the amount of fecal oocysts in the drug-treated mice was significantly decreased compared with that in the positive control group (P <0.05). The intestinal pathology showed that the small intestine epithelium was under repair. After drug treatment, CD28 expression in all treatment groups was significantly higher than that in positive control group B1 (P <0.05), C2 in psoralen treatment group was the highest (64.01 ± 5.39), significantly higher than that in dihydroartemisinin treatment group D2 (P <0.05). Conclusion Psoralen and dihydroartemisinin can inhibit Cryptosporidium, of which dihydroartemisinin has obvious insecticidal effect. Psoralen mainly regulates the proportion of CD28 molecules in peripheral blood and the ratio of CD4 + to CD3 + T cells, Through synergy, they participate in the host immune response and inflammatory reaction process, and eventually repair the inflammatory reaction.