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目的观察IL-6对1-甲基-4-苯基吡啶(MPP+)处理的SH-SY5Y细胞的生长和pERK的影响。方法对MPP+处理的SH-SY5Y细胞进行IL-6干预,观察细胞结构形态的改变以及pERK的含量变化和定位。结果 MPP+处理的SH-SY5Y细胞系细胞活力下降;细胞系pERK上升,高峰在24h,主要定位于胞浆;IL-6干预的SH-SY5Y细胞系pERK上升,高峰在6h,多定位于胞核。IL-6可以降低MPP+处理的SH-SY5Y细胞系的凋亡,使pERK分泌高峰提前;加用ERK抑制剂U0126可以下调IL-6对MPP+处理的SH-SY5Y细胞系的影响。结论IL-6可以通过调节pERK,减少MPP+诱导的细胞损伤,促进SH-SY5Y细胞的存活。
Objective To investigate the effects of IL-6 on the growth and pERK of SH-SY5Y cells treated with 1-methyl-4-phenylpyridine (MPP +). Methods Interleukin-6 (IL-6) intervention was performed on SH-SY5Y cells treated with MPP +, and the changes of cell morphology and the changes of pERK contents and localization were observed. Results The cell viability of SH-SY5Y cells treated with MPP + decreased. The expression of pERK in the cell line increased at a peak at 24 h and mainly localized in the cytoplasm. The pERK of SH-SY5Y cell line increased with the increase of 6 h . IL-6 can reduce the apoptosis of SH-SY5Y cells treated with MPP + and lead to the peak of pERK secretion. The addition of ERK inhibitor U0126 can down-regulate the effect of IL-6 on MPP + -treated SH-SY5Y cells. Conclusion IL-6 can promote the survival of SH-SY5Y cells through regulating pERK, reducing MPP + -induced cell injury.