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目的探讨黄芪注射液(AMI)是否通过调节糖皮质激素代谢影响同种移植物生存。方法体内应用AMI后,逐日观察同种移植小鼠移植物生存状况;应用受体放射配基结合分析测定脾细胞糖皮质激素受体(GR)结合位点;利用放射免疫分析检测血清皮质醇水平。结果同种移植早期小鼠脾细胞GR结合位点数和血清皮质醇明显升高,此后逐渐下降,在排斥高峰期(第14天)达到最低值。单用AMI(60 g/kg)或环磷酰胺(CTX,100 mg/kg)及二者联合应用均可显著延长同种移植物的生存时间。AMI单独或与CTX联合应用可以明显上调排斥高峰期受体小鼠脾细胞GR位点数和血清皮质醇水平,AMI可以逆转CTX引起的GR结合位点数的下降(P<0.05)。结论 AMI可明显抑制同种移植排斥反应,与CTX联用可明显提高移植物存活率,其作用机制可能与上调GR结合位点数和血清皮质醇水平,促进内源性糖皮质激素发挥作用有关。
Objective To investigate whether astragalus injection (AMI) affects the survival of allografts by regulating glucocorticoid metabolism. Methods The survival of allograft mice was observed daily with AMI in vivo. The binding sites of glucocorticoid receptor (GR) in spleen cells were determined by radioligand binding assay. The level of serum cortisol was measured by radioimmunoassay . Results The number of GR binding sites and serum cortisol in splenocytes of mice in early allotransplantation increased significantly, then decreased gradually and reached the lowest value at the peak of repulsion (day 14). A single use of AMI (60 g / kg) or cyclophosphamide (CTX, 100 mg / kg) and a combination of both significantly prolonged the allograft survival time. AMI alone or in combination with CTX significantly up-regulated the splenocyte GR locus and serum cortisol level in peak recipient mice, and AMI reversed the decrease in CTX-induced GR binding sites (P <0.05). Conclusions AMI can significantly inhibit the allograft rejection. Combined with CTX can significantly improve the graft survival rate. The mechanism may be related to up-regulating the number of GR binding sites and serum cortisol and promoting the effect of endogenous glucocorticoids.