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目的 :建立金黄色葡萄球菌随机扩增DNA多态性 (RAPD)分型技术 ,以应用于医院感染流行病学调查。方法 :对临床分离的 85株金黄色葡萄球菌 ,用酚氯仿法提取DNA后进行RAPD分型。结果 :临床分离株大多数产生独特而稳定的RAPD带型 ,不同医院的基因型不同 ,同一医院基因型相似 ,在同一医院某些病区金黄葡萄球菌医院感染可能为交叉感染。结论 :RAPD基因分型技术不需预先知道核酸序列 ,分型率高 ,分辨力强 ,简便快捷 ,可为金黄色葡萄球菌临床分离株提供分型标志 ,是分子流行病学研究的有效方法。
Objective: To establish random amplified polymorphic DNA (RAPD) typing of Staphylococcus aureus for epidemiological investigation of nosocomial infection. Methods: Eighty-five Staphylococcus aureus isolates were isolated by RAPD using phenol-chloroform method. Results: Most of the clinical isolates produced unique and stable RAPD patterns. Different genotypes were different in different hospitals. Genotypes in the same hospital were similar. Nosocomial infections with S. aureus in some wards of the same hospital may be cross-infection. Conclusion: The RAPD genotyping technique does not need to know the nucleic acid sequence in advance. It has high typing rate, strong resolving power, simple and quick method, and can provide typing marks for Staphylococcus aureus clinical isolates. It is an effective molecular epidemiological study.