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青霉(Penicillium)、曲霉(Aspergillum)和放线菌(Actinomycetes)是中学植物实验课必需的实验材料。本人经过摸索,为中学制作出霉菌、放线菌活体悬滴标本和永久装片,很受中学老师们的欢迎,在中学简陋条件下很易制作,效果极佳,现介绍如下。 1.悬滴活标本的制作方法 (1)倒平板 100毫升水中加2克琼脂,煮开使琼脂溶化,待凉至40℃左右(不烫手为度),将该液态琼脂培养基倒入直径90毫米的培养皿中,每皿倒培养基15—20毫升左右,约2—3毫米厚。待琼脂凝固(5—10分钟左右)
Penicillium, Aspergillum, and Actinomycetes are necessary experimental materials for high school plant experiments. I have explored for secondary schools to produce mold, actinomycetes in vivo hanging drop specimens and permanent loading, very popular with high school teachers, very simple conditions in secondary school is very easy to produce, the effect is excellent, are presented below. 1. hanging drops live specimens of production methods (1) inverted plate 100 ml of water plus 2 g of agar, boil the agar dissolved until cool to about 40 ℃ (not hot for the degree), the liquid agar medium into the diameter In a 90 mm Petri dish, pour about 15-20 ml of medium per dish, about 2-3 mm thick. Until agar coagulation (5-10 minutes or so)