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体内重建试验证明,骨髓来源的 Pro-T 细胞,在胸腺内经历多阶段的发育,分化为对自身抗原耐受、对外来抗原应答的功能成熟 T 细胞,迁出胸腺.经嵌合小鼠及转基因小鼠研究证明,胸腺微环境基质细胞(TSC)对胸腺内 T 细胞的发育,有选择作用,阳性选择导致 T 细胞功能发育,阴性选择导致 T 细胞克隆消除或不活化.鉴于 TSC 类型的多样性,很难分析胸腺内起选择作用的细胞类型及分子机理.为此,体外建立 TSC 系,在体外培养中,分析已知类型的 TSC 对特定发育阶段的 T 细胞分化的作
In vivo reconstitution test proves that bone marrow-derived Pro-T cells undergo multiple stages of development in the thymus and differentiate into mature T cells that are resistant to their own antigens and respond to foreign antigens and migrate out of the thymus. Studies in transgenic mice have shown that thymic microenvironment stromal cells (TSC) have a selective role in the development of T cells in the thymus, positive selection leads to the development of T cell function, and negative selection leads to the elimination or inactivation of T cell clones. Given the diversity of TSC types It is difficult to analyze the cell type and molecular mechanism of the selective function in the thymus.Therefore, TSC line was established in vitro, and in vitro culture, analysis of the known type of TSC on T cell differentiation at a specific developmental stage