论文部分内容阅读
目的:探讨中药肺岩宁方含药血清对人肺癌细胞系A549细胞增殖及肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)诱导的A549细胞分泌白细胞介素6(interleukin-6 ,IL-6)和IL-8的影响。方法:肺岩宁方含药血清培养A549细胞24、48和72 h后,采用四唑单钠盐法检测肺岩宁方含药血清对A549细胞增殖的影响;肺岩宁方含药血清培养A549细胞48 h后,酶联免疫吸附测定法检测肺岩宁方含药血清对TNF-α诱导的IL-6和IL-8分泌的影响,逆转录聚合酶链式反应检测IL-6和IL-8 mRNA表达水平的变化。结果:肺岩宁方含药血清对A549细胞增殖具有明显的抑制作用。1μg/L TNF-α可明显提高A549细胞上清液中IL-6和IL-8的分泌水平,肺岩宁方含药血清与TNF-α共同作用后,可抑制IL-6和IL-8的分泌(P<0 .01)及IL-6、IL-8 mRNA的表达(P<0 .01)。结论:肺岩宁方含药血清对TNFα诱导A549细胞分泌IL-6和IL-8具有明显的抑制作用,这为其临床治疗肺癌提供了一定的实验依据。
OBJECTIVE: To investigate the effects of the Chinese herbal medicine Feiyanningfang serum on the proliferation of human lung cancer cell line A549 and the effect of TNF-α on the secretion of interleukin-6 (IL) -6) and IL-8. METHODS: A549 cells were treated with Feiyanningfang serum for 24,48 and 72 h. The effect of Feiyanningfang serum on proliferation of A549 cells was detected by tetrazolium monosodium salt method. A549 cells 48 h later, the enzyme-linked immunosorbent assay was used to detect the effect of Feiyanningfang serum on the secretion of IL-6 and IL-8 induced by TNF-α, and the levels of IL-6 and IL-6 by reverse transcription polymerase chain reaction -8 mRNA expression level changes. Results: Feiyanningfang serum could significantly inhibit the proliferation of A549 cells. 1μg / L TNF-α can significantly increase the secretion of IL-6 and IL-8 in the supernatant of A549 cells, and the combination of Feiyanning Fang serum and TNF-α can inhibit IL-6 and IL-8 (P <0.01) and IL-6, IL-8 mRNA expression (P <0.01). Conclusion: Feiyanningfang serum can significantly inhibit the secretion of IL-6 and IL-8 by A549 cells induced by TNFα, which provides some experimental evidences for the clinical treatment of lung cancer.