论文部分内容阅读
目的:研究翠绿针毛蕨Macrothelypteris viridifrons的化学成分及其抑制肿瘤细胞增殖作用。方法:利用硅胶、C18反相硅胶、Sephadex LH-20凝胶等色谱技术对其提取物进行分离纯化,根据化合物的理化性质和波谱数据进行结构鉴定,以MTT法测试各化合物对MOLT-4,Hep G2,A-549,MCF-7,HT-29,PC-3肿瘤细胞增殖的抑制作用。结果:分离得到5个具有特殊B环结构的黄酮类化合物,分别鉴定为:原芹菜素(1),4’-羟基-原芹菜素(2),4’-羟基-原芹菜素4’-O-β-D-葡萄糖苷(3),5,7-二羟基-2-(1,2-异丙二氧基-4-酮-环己-5-烯)-色原酮(4),5,7-二羟基-2-(1-羟基-2,6-二甲氧基-环己-4-酮)-色原酮(5)。结论:所有化合物均首次从翠绿针毛蕨中分离得到,化合物1,4和5对6种人肿瘤细胞增殖有明显的抑制作用,且呈现出良好的剂量依赖性。
Objective: To study the chemical constituents of Macrothelypteris viridifrons and their inhibitory effect on the proliferation of tumor cells. Methods: The extracts were separated and purified by chromatography on silica gel, C18 reversed-phase silica gel and Sephadex LH-20 gel. The structures of the extracts were elucidated on the basis of physicochemical properties and spectral data. The inhibitory effect of MOLT-4, Hep G2, A-549, MCF-7, HT-29, PC-3 tumor cell proliferation. Results: Five flavonoids with special B ring structure were isolated and identified as: apigenin (1), 4’-hydroxy-proereptin (2), 4’-hydroxy- O-β-D-glucoside (3), 5,7-dihydroxy-2- (1,2-isopropoxydioxy- , 5,7-dihydroxy-2- (1-hydroxy-2,6-dimethoxy-cyclohexan-4-one) -chromanone (5). CONCLUSION: All compounds were isolated for the first time from Pinctada fern. Compounds 1, 4 and 5 inhibited the proliferation of six human tumor cells in a dose-dependent manner.