为探明芽变雄花序赤霉素含量降低的分子机制,对板栗野生和芽变雄花序赤霉素合成关键酶基因古巴焦磷酸合成酶(CPS)、贝壳杉烯合成酶(KS)、贝壳杉烯氧化酶(KO)、贝壳杉烯酸氧化酶(KAO)、赤霉素20-氧化酶1(GA20ox1)和赤霉素3-氧化酶1(GA3ox1)的cDNA序列,以及KO基因DNA序列进行了比对,结果表明:两类花序中KO基因在开放阅读框的872、1 115和1 150bp存在3处碱基差异,分别造成了氨基酸由Glu、Tyr和Tyr突变为Gly、Phe和His,最终导致了跨膜区的改变,且发现cDNA上碱基突变是由DNA的碱基差异造成的。推测KO基因的这种突变导致板栗短雄花序性状。 In order to explore the molecular mechanism of the decrease of maleicang gibberellin in buds, the effects of cp, ks, (KO), KAO, GA20ox1 and GA3ox1, and the cDNA sequence of KO gene The results showed that there were 3 base differences between KO gene in 872, 115 and 1 150 bp in open reading frame (ORF) of two types of inflorescences, which resulted in the mutation of Glu, Tyr and Tyr to Gly, Phe and His , Eventually leading to changes in the transmembrane region, and found that the base mutation in the cDNA is caused by the base difference of DNA. This mutation of the KO gene is presumed to result in the short chestnut male inflorescence trait.