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电磁场对健康影响的研究包括流行病调查、人体与动物、细胞、生化与分子生物、生物物理等5个层次,电磁生物效应最初是通过物理作用产生化学反应,继而产生后续生物反应.自由基、电磁能量和生物钙是分属于化学、物理学和生物学的3个概念,研究它们之间的关系对于认识电磁生物效应的原初作用具有意义.选择海马神经元,观察在0.1mT、0.5mT和1.0mT电磁场暴露48h,海马神经元ROS水平和胞内Ca2+浓度的变化.实验结果表明:暴露于0.1mT,0.5mT和1.0mT电磁场海马神经元的ROS水平和Ca2+浓度都比对照组有显著性提高(P<0.01).暴露于0.1mT和0.5mT电磁场的ROS水平和暴露于0.1mT电磁场的Ca2+浓度与自由基清除剂+电磁场(Trolox+EMF)组比较没有差异(P>0.05),暴露于1.0mT电磁场的ROS水平和暴露于0.5mT和1.0mT电磁场的Ca2+浓度比Trolox+EMF组有显著性提高(P<0.01).表明电磁场可以促进细胞自由基的产生,并且ROS水平与胞内Ca2+浓度有正相关性.
The research on the health effects of electromagnetic fields includes epidemiological investigation, human and animal, cell, biochemistry and molecular biology, and biological physics. The electromagnetic biological effects firstly produce chemical reaction through physical action and then produce subsequent biological reaction.Free radicals, Electromagnetic energy and bio-calcium belong to the three concepts of chemistry, physics and biology, and studying the relationship between them is of great significance for understanding the primary role of electromagnetic bio-effect.Selecting hippocampal neurons, we observe the effects of 0.1mT, 0.5mT and The changes of ROS level and intracellular Ca2 + concentration in hippocampal neurons exposed to 1.0mT EMF for 48h.The experimental results showed that the levels of ROS and Ca2 + in hippocampal neurons exposed to 0.1mT, 0.5mT and 1.0mT electromagnetic fields were significantly higher than those in control group (P <0.01) .The level of ROS exposed to 0.1mT and 0.5mT electromagnetic fields and Ca2 + exposed to 0.1mT electromagnetic field showed no difference (P> 0.05) compared with the group of free radical scavenger + electromagnetic field (Trolox + EMF) ROS level in 1.0mT electromagnetic field and Ca2 + concentration in electromagnetic field exposed to 0.5mT and 1.0mT were significantly higher than those in Trolox + EMF group (P <0.01), indicating that electromagnetic field can promote the generation of cell free radicals, And ROS levels and intracellular Ca2 + concentration has a positive correlation.