论文部分内容阅读
目的 应用双色流式细胞仪动态检测 10例恶性血液病病人外周血干细胞移植 (PBSCT)治疗前后 ,外周血淋巴细胞免疫表型及其临床意义。方法 10例PBSCT病例中 ,7例PBSCT ,男性 3例 ,女性 4例 ;年龄 2 1～ 4 2岁 ,4例ANLL -M2 ,1例CML ,2例淋巴瘤 ,3例为异基因外周血干细胞移植 (allo -PBSCT) ,男性 2例 ,女性 1例 ;年龄 37～ 4 6岁 ,CML ,ANLL -M2 ,ANLL -M3 各 1例。结果 10例病人移植前已出现淋巴细胞表型异常 ,其中以代表T抑制细胞 (Ts)的CD3 + CD8+ 水平增高 ,Th/Ts值下降 ,代表B细胞的CD3 -,CD19+ 水平明显降低。移植后 3个月 :CD3 + CD4+ :(16 .5± 7.5 ) % ,CD3 + D8+ :(5 5 .3± 2 3) % ,Th/Ts:0 .2 7±0 .18,CD3 -CD19-L :未测出 ,CD16+ /CD56+ :(14 .3± 9.5 ) %。移植前后的淋巴细胞 (T +B +NK)、T细胞(CD3 + CD19-)及NK细胞 (CD16+ /CD56+ )的百分率水平上没有明显变化 (P >0 .0 5 ) ,但在其构成比例上有异常 ,特别是CD3 + CD4+ 水平降低 ,CD3 + CD8+ 水平升高 ,Th/Ts比值及CD3 -CD19+ 水平均有明显降低 (P <0 .0 5 )。B细胞在 6个月左右开始恢复 ,Th(CD3 + CD4+ )、Ts(CD3 + CD8+ )及Th/Ts恢复很慢 ,个别病例 1年后仍未恢复正常水平。 10例PBSCT后有 4例病人出现不同程度的感染 ,均发生在?
Objective To detect the immunophenotypes of peripheral blood lymphocytes by using two-color flow cytometry in 10 patients with hematologic malignancies before and after PBSCT treatment and its clinical significance. METHODS: Ten PBSCT patients were treated with PBSCT in 7 cases. There were 3 males and 4 females. Aged 21 to 42, 4 ANLL-M2, 1 CML, 2 lymphoma and 3 allogeneic peripheral blood stem cells Transplantation (allo-PBSCT) was performed in 2 males and 1 females. CML, ANLL-M2 and ANLL-M3 were each aged from 37 to 46 years old. Results Ten patients had abnormal lymphocyte phenotype before transplantation, in which the level of CD3 + CD8 + was increased and the Th / Ts was decreased on behalf of T suppressor cells (Ts), which markedly decreased the level of CD3 - and CD19 + in B cells. Three months after transplantation, CD3 + CD4 +: (16.5 ± 7.5)%, CD3 + D8 +: (53.3 ± 23)%, Th / Ts: 0.27 ± 0.18, -L: Not detected, CD16 + / CD56 +: (14.3 ± 9.5)%. The percentage of T lymphocytes (T + B + NK), T cells (CD3 + CD19-) and NK cells (CD16 + / CD56 +) before and after transplantation did not change significantly (P> 0.05) Especially CD3 + CD4 +, CD3 + CD8 +, Th / Ts ratio and CD3-CD19 + levels were significantly lower (P <0.05). B cells began to recover at about 6 months. The recovery rate of Th (CD3 + CD4 +), Ts (CD3 + CD8 +) and Th / Ts was very slow. Four of the 10 patients after PBSCT showed varying degrees of infection, both in?