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利用静电纺丝纳米纤维具有高比表面积和多孔疏松结构的优势,固定葡萄糖淀粉酶(GA)以提高酶的重复使用性。采用活化酯法,以甲苯基磺酰氟(PMSF)为基团活化剂在乙醇溶液中活化聚乙烯醇(PVA)纳米纤维膜,再在水溶液以共价键连接固定GA。通过对红外光谱的分析,表明GA可成功固定在静电纺丝形成的PVA纳米纤维膜表面。与游离的GA相比较,固定化的GA体现出较高的酶活性,更宽泛的适用温度和pH,以及更好的热稳定性和存贮稳定性,具有较好的重复使用性。此法制备生物活性酶膜操作简单,具有一定的应用前景。
Utilizing the advantages of electrospun nanofibers with high specific surface area and porous, porous structures, glucoamylase (GA) is immobilized to enhance enzyme reusability. Activated ester method was used to activate polyvinyl alcohol (PVA) nanofibrous membrane in ethanol solution with toluene sulfonyl fluoride (PMSF) as activator. Then, GA was covalently immobilized in aqueous solution. Through the analysis of infrared spectrum, GA can be successfully fixed in the electrospinning PVA nanofiber membrane surface. Compared with the free GA, the immobilized GA showed higher enzyme activity, broader applicable temperature and pH, better thermal stability and storage stability, and better reusability. The method for preparing bioactive enzyme membrane has the advantages of simple operation and certain application prospect.