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目的：研究卡介苗（BCG）65KD热休克蛋白的提纯方法。方法：BCG经37℃恒温振荡培养两周，42℃热休克处理2h，培养液经离心、抽滤、盐析沉淀、SDS-PAGE分离、电泳洗脱等步聚纯化。结果：提纯产物经SDS－PAGE分析，其电泳区带在65KD位置，经抑制试验表明具有较高抗原活性结论：流程方法简单、实用，有助于对IDDM的诊断及其深入研究。
Objective: To study the purification method of BCG 65KD heat shock protein. Methods: BCG was incubated at 37 ℃ for two weeks with heat shock at 42 ℃ for 2 h. The medium was centrifuged, suction filtered, salted out, separated by SDS-PAGE and eluted by electrophoresis. Results: The purified product was analyzed by SDS-PAGE. The electrophoresis band was at 65KD. The inhibitory test showed that the purified product had high antigenic activity. Conclusion: The method is simple and practical, which is helpful for the diagnosis of IDDM and its further study.