论文部分内容阅读
目的:确定角质细胞生长因子受体(KGFR)在正常宫颈组织、宫颈癌组织中有无表达;了解KGFR对Hela、CaSki细胞增殖有无影响。方法:①采用免疫组织化学方法对正常宫颈组织(22例)和宫颈癌组织(31例)中KGFR的表达进行测定,比较正常宫颈组织和宫颈癌组织中KGFR的表达有无差异;②3H-TdR掺入试验测定KGFR抗体对Hela、CaSki细胞增殖的影响。结果:①正常宫颈上皮细胞无KGFR表达,但宫颈癌上皮细胞中KGFR表达明显,KGFR在宫颈癌上皮细胞中的表达率为71%;在Ⅱa期宫颈癌中KGFR的阳性表达率高于原位癌(P<0.05),但Ⅰb期宫颈癌与原位癌及Ⅰb期和Ⅱa期宫颈癌中KGFR的表达在统计学上无差异(P>0.05)。宫颈癌出现深部间质浸润时KGFR的阳性表达率高于浅部间质浸润者(P<0.05);②在加入2μg/mlKGFR抗体后Hela、CaSki细胞的增殖均减弱,与对照组相比存在差异(P<0.05)。结论:KGFR与宫颈癌的增殖、侵袭有关。在宫颈癌的发生发展中KGFR可能起了一定作用。
Objective: To determine the expression of keratinocyte growth factor receptor (KGFR) in normal cervical tissues and cervical cancer tissues. To investigate whether KGFR affects the proliferation of Hela and CaSki cells. Methods ① Immunohistochemistry was used to detect the expression of KGFR in normal cervical tissue (22 cases) and cervical cancer tissue (31 cases), and to compare the expression of KGFR in normal cervical tissue and cervical cancer tissue; (2) 3H-TdR The effect of KGFR antibody on proliferation of Hela and CaSki cells was determined by incorporation assay. Results: ①The normal cervical epithelial cells had no KGFR expression, but the expression of KGFR was significantly higher in cervical epithelial cells. The positive expression rate of KGFR in cervical epithelial cells was 71%. The positive expression rate of KGFR in cervical carcinoma Ⅱa was higher than that in situ (P <0.05). However, there was no statistical difference in the expression of KGFR between stage Ⅰb cervical cancer and carcinoma in situ and in stage Ⅰb and Ⅱa cervical cancer (P> 0.05). The positive rate of KGFR expression in cervical cancer was higher than that in superficial interstitial infiltration in deep interstitial infiltration (P <0.05); ② The proliferation of Hela and CaSki cells were weakened after 2μg / ml KGFR antibody was added, compared with the control group Difference (P <0.05). Conclusion: KGFR is associated with the proliferation and invasion of cervical cancer. KGFR may play a role in the development of cervical cancer.