论文部分内容阅读
目的探讨大气PM2.5对哮喘患者炎症因子的影响,寻找用于评价PM2.5污染效应的敏感指标,为阐明PM2.5加重哮喘发生的机制提供参考。方法选择太原市迎泽区社区哮喘患者19名,分别于2012年12月和2013年6月对患者进行7 d的大气PM2.5暴露监测,并于监测最后一天采集血样进行炎症因子检测,比较两阶段哮喘患者的PM2.5暴露差别及体内相关炎症因子[白介素-8(IL-8)、白介素-13(IL-13)、γ干扰素(IFN-γ)]的变化情况。结果哮喘患者冬季室外、室内大气PM2.5暴露日均浓度和个体PM2.5暴露日均浓度均高于夏季;冬季血浆炎症因子IL-13浓度高于夏季,IFN-γ浓度低于夏季,差异均有统计学意义(P<0.05)。哮喘患者个体PM2.5暴露浓度与室外、室内PM2.5暴露浓度呈正相关(r值分别为0.809,0.826,P<0.05),室外PM2.5暴露浓度与室内PM2.5暴露浓度呈正相关(r=0.769,P<0.05)。哮喘患者血浆IL-8浓度与个体PM2.5暴露浓度呈正相关(r=0.498,P<0.05);血浆IL-13与室外PM2.5暴露浓度呈正相关(r=0.579,P<0.05),与IFN-γ浓度呈负相关(r=-0.536,P<0.05)。结论外周血中炎症因子IL-13、IFN-γ可能是大气PM2.5加重哮喘的敏感指标。
Objective To investigate the effect of PM2.5 on inflammatory cytokines in asthmatic patients and to find sensitive indicators for assessing the effect of PM2.5 pollution in order to provide a reference for elucidating the mechanism of PM2.5 exacerbating asthma. Methods Twenty-nine patients with community asthma in Yingze District, Taiyuan City were enrolled. Patients were exposed to PM2.5 in the atmosphere for 7 days in December 2012 and June 2013 respectively. Blood samples were collected on the last day of monitoring to detect inflammatory cytokines. PM2.5 exposure in two-stage asthmatic patients and the changes of inflammatory cytokines (interleukin-8, interleukin-13, interferon-gamma) in vivo. Results In winter, indoor air PM2.5 exposure daily average concentration and individual PM2.5 daily exposure concentration were higher in asthma patients than in summer. In winter, plasma inflammatory cytokines IL-13 concentrations were higher than those in summer and IFN-γ concentrations were lower than those in summer All were statistically significant (P <0.05). The PM2.5 exposure level in asthmatic patients was positively correlated with the indoor and outdoor exposure concentrations of PM2.5 (r = 0.809, 0.826, respectively, P <0.05). The outdoor PM2.5 exposure concentration was positively correlated with the indoor exposure concentration of PM2.5 = 0.769, P <0.05). Plasma IL-8 levels in asthmatic patients were positively correlated with PM2.5 exposure (r = 0.498, P <0.05). Plasma IL-13 levels were positively correlated with PM2.5 exposure (r = 0.579, P <0.05) IFN-γ concentration was negatively correlated (r = -0.536, P <0.05). Conclusions Inflammatory cytokines IL-13 and IFN-γ in peripheral blood may be sensitive indicators of atmospheric PM2.5 aggravating asthma.