目的 :检测雏鸡视网膜褪黑激素的含量。方法 :采用高效液相色谱法。结果 :方法操作简单 ,结果可信 ,检出限为2 5ng/ml。线性范围 0 0 2 5～ 80 μg/ml,变异系数 <1 1 % (日内、日间 )。加标回收率 82 0 %～ 99 8% ,样品中褪黑激素含量0 2 μg/ml。结论 :出生 1～ 7d雏鸡松果体不分泌褪黑激素。 Objective: To detect the content of retinal melatonin in chickens. Methods: Using high performance liquid chromatography. Results: The method was simple and credible with the detection limit of 25ng / ml. The linear range was 0 0 2 5 ~ 80 μg / ml with a coefficient of variation <1 1% (intraday, daytime). The spiked recoveries ranged from 82% to 99.8%. The content of melatonin in the samples was 0.2 μg / ml. Conclusion: Chickens from 1 to 7 days old do not secrete melatonin.