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建立了一种基于C-Ag~+-C配位作用和核酸三链体系的表面增强拉曼光谱方法以快速检测Ag~+。单链DNA(ssDNA)能够分散和稳定修饰有罗丹明6G的金纳米颗粒。当加入Ag~+后,该富含胞嘧啶(C)的ssDNA通过Ag~+配位作用与dsDNA形成具有刚性结构的三链DNA(tsDNA),tsDNA无法稳定金纳米颗粒,致使其发生团聚而产生R6G的拉曼信号峰,实现检测Ag~+的目的。实验对制备的金纳米颗粒进行了表征,并考察了DNA浓度对金纳米颗粒稳定性的影响。结果显示,在优化的条件下,相比于其它11种金属离子,本法对Ag~+具有较强的选择性。在1~10μmol/L范围内,拉曼峰强度和Ag~+浓度的线性关系良好,相关系数R为0.9988。方法适用于Ag~+的快速分析。
A surface-enhanced Raman spectroscopy method based on C-Ag ~ + -C coordination and triplex system of nucleic acid was developed to detect Ag ~ + rapidly. Single-stranded DNA (ssDNA) is capable of dispersing and stably modifying gold nanoparticles with rhodamine 6G. When Ag + was added, the cytosine (C) -rich ssDNA formed a rigid three-stranded DNA (tsDNA) with dsDNA through Ag ~ + coordination, and tsDNA failed to stabilize the gold nanoparticles, causing them to agglomerate Raman signal generated R6G peak, to achieve the purpose of detecting Ag ~ +. The prepared gold nanoparticles were characterized experimentally, and the effect of DNA concentration on the stability of gold nanoparticles was investigated. The results show that this method is more selective for Ag ~ + than other 11 kinds of metal ions under optimized conditions. In the range of 1 ~ 10μmol / L, the linear relationship between Raman intensity and Ag ~ + concentration was good, with a correlation coefficient R of 0.9988. The method is suitable for the rapid analysis of Ag ~ +.