论文部分内容阅读
This paper reports the results of gossypol effects on the testicular mitochondrial ultrastructures and functions. A hypothesis of mechanism of gossypol action based on our experimental data was postulated.Electron microscopic observations confirmed further our previous studies that the mitoehondria of the target germ cells were the most sensitive and the most severely damaged among cellular organelles in response to gossypol. The damages included the swelling, vacuolation, crista depletion, lysis, granular accumulation in matrix and the process of intact mitochondria disintegration. The activity of the mitochondrial marker enzyme, the LDH-X of human spermatozoa, was markedly decreased or suppressed completely after gossypol treatment.The functions of isolated testieular mitochondria determined by Warburg manometric and oxygen electrode polarographic methods indicated that gossypol stimulated respiration but inhibited slightly the oxidative phosphorylation at low concentrations (20-40μM) in vitro. With
This paper reports the results of gossypol effects on the testicular mitochondrial ultrastructures and functions. A hypothesis of mechanism of gossypol action based on our experimental data was postulated. Electron microscopic observations confirmed further previous previous that that the mitoehondria of the target germ cells were the most The activity of the mitochondrial marker enzyme, the LDH- X of human spermatozoa, marked or decreased completely after oxidized phosphorylation at low concentrations M) in vitro. With