建立了高效薄层扫描法同时测定复方维甲酸霜中维甲酸和维生素Ｅ含量的方法。以正己烷：乙醚：甲酸（９：１：０．１）为展开剂。采用双波长反射锯齿法，测维甲酸含量时λ＿ｓ＝３５０ｎｍ，λ＿Ｒ＝２９５ｎｍ，测维生素Ｅ含量时λ＿ｓ＝２８０ｎｍ，λ＿Ｒ＝３２０ｎｍ。加样回收率维甲酸为９９．２％（ＲＳＤ２．０％），维生素Ｅ为９７．０％（ＲＳＤ１．２％）。本文就不同时间及不同贮存条件下霜剂的含量进行了测定，结果室温时随放置时间的延长，主药含量均有明显的下降。 A high performance thin layer scanning method was established for simultaneous determination of retinoic acid and vitamin E in the compound tretinoin creams. With n-hexane: ether: formic acid (9: 1: 0.1) as developing solvent. Using dual wavelength sawtooth method, the measured retinoic acid content λ_s = 350nm, λ_R = 295nm, measured vitamin E content when λ_s = 280nm, λ_R = 320nm. The recovery rate of retinoic acid was 99.2% (RSD 2.0%) and vitamin E was 97.0% (RSD 1.2%). In this paper, different time and different storage conditions cream content were determined, the results at room temperature with the extension of time, the main drug content decreased significantly.