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采用逆转录-筑巢式聚合酶链反应(nestedPCR)技术检测33例不同临床阶段的慢性粒细胞白血病(CMu及18例急性淋巴细胞白血病(ALL)患者的bcr/ablmRNA。结果:33例CML中31例阳性,其中21例表达bcrexon3/ablexon2mRNA,7例表达bcrexon2/ablexon2mRNA,2例同时表达这两种mRNA。在18例ALL中阳性6例,其中4例表达hrexon3/ablexon2mRNA,2例表达bcrexon3/ablexon2+bcrexonl/ablexon2mRNA。本实验的敏感度达10 ̄(-6)~10 ̄(-7)水平,有助于临床上白血病微量残留病的早期诊断。
Thirty-three cases of chronic myeloid leukemia (CMu) and 18 cases of acute lymphoblastic leukemia (ALL) were examined for bcr/abl mRNA by reverse transcription-nesting polymerase chain reaction (PCR). Results: 33 cases of CML Thirty-one patients were positive, of which 21 expressed bcrexon3/ablexon2 mRNA, 7 expressed bcrexon2/aablexon2 mRNA, and 2 expressed both mRNA simultaneously.Among 6 patients with ALL, 6 were positive, of which 4 expressed hrexon3/ablexon2 mRNA and 2 expressed bcrexon3/ Ablexon2+bcrexonl/ablexon2mRNA.The sensitivity of the experiment reached 10 ~ (-6) ~ 10 ~ (-7) level, which contributes to the early diagnosis of leukemia minimal residual disease.