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采用逆转录－筑巢式聚合酶链反应（ｎｅｓｔｅｄＰＣＲ）技术检测３３例不同临床阶段的慢性粒细胞白血病（ＣＭｕ及１８例急性淋巴细胞白血病（ＡＬＬ）患者的ｂｃｒ／ａｂｌｍＲＮＡ。结果：３３例ＣＭＬ中３１例阳性，其中２１例表达ｂｃｒｅｘｏｎ３／ａｂｌｅｘｏｎ２ｍＲＮＡ，７例表达ｂｃｒｅｘｏｎ２／ａｂｌｅｘｏｎ２ｍＲＮＡ，２例同时表达这两种ｍＲＮＡ。在１８例ＡＬＬ中阳性６例，其中４例表达ｈｒｅｘｏｎ３／ａｂｌｅｘｏｎ２ｍＲＮＡ，２例表达ｂｃｒｅｘｏｎ３／ａｂｌｅｘｏｎ２＋ｂｃｒｅｘｏｎｌ／ａｂｌｅｘｏｎ２ｍＲＮＡ。本实验的敏感度达１０￣（－６）～１０￣（－７）水平，有助于临床上白血病微量残留病的早期诊断。
Thirty-three cases of chronic myeloid leukemia (CMu) and 18 cases of acute lymphoblastic leukemia (ALL) were examined for bcr/abl mRNA by reverse transcription-nesting polymerase chain reaction (PCR). Results: 33 cases of CML Thirty-one patients were positive, of which 21 expressed bcrexon3/ablexon2 mRNA, 7 expressed bcrexon2/aablexon2 mRNA, and 2 expressed both mRNA simultaneously.Among 6 patients with ALL, 6 were positive, of which 4 expressed hrexon3/ablexon2 mRNA and 2 expressed bcrexon3/ Ablexon2+bcrexonl/ablexon2mRNA.The sensitivity of the experiment reached 10 ~ (-6) ~ 10 ~ (-7) level, which contributes to the early diagnosis of leukemia minimal residual disease.