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单克隆抗体（ｍＡｂ）是一类均一性很好的蛋白质，但由于不同ｍＡｂ的理化特性有差异，纯化方法和条件也有所不同。本文通过反复实验，摸索出利用阳离子色谱介质可较好地纯化小鼠抗重组人ＴＮＦ－αｍＡｂＦ６（ＩｇＧ１）的实验条件。１ 材料和方法１－１ 主要试剂 小鼠抗
Monoclonal antibodies (mAbs) are a class of very homogeneous proteins, but due to the differences in the physicochemical properties of the different mAbs, the methods and conditions of purification are different. In this paper, repeated experiments were conducted to find out the experimental conditions for the purification of mouse anti-recombinant human TNF-α mAbF6 (IgG1) by cation chromatography. 1 Materials and Methods 1-1 The main reagent mouse anti