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目的 探讨一氧化氮 (NO)与精子运动能力的关系。方法 采用镀铜镉还原荧光法 ,测定人精液中NO代谢产物硝酸盐 (NO3 -)。参照WHO方法 ,在超高倍显微镜下观察精子存活率、活动力等。结果 80例男性不育者其中 15例NO浓度明显低于正常对照组精子活动力a +b级 <5 0 % (P <0 .0 1) ,以运动轨迹异常和头摆动幅度下降为主 ,精子存活力 >75 %无明显差异 (P >0 .0 5 ) ;6 5例NO浓度显著高于正常对照精子活动力a +b级 <5 0 % ,存活率 <75 % (P <0 .0 0 1)。结论 NO与精子运动功能有着密切关系。高浓度时明显抑制精子活动力及存活率。低浓度时精子存活率及功能有着维护作用 ,这对男性不育的病因研究和治疗有非常重要的临床价值。
Objective To investigate the relationship between nitric oxide (NO) and sperm motility. Methods Cadmium - cadmium reduction fluorescence method was used to determine NO metabolite nitrate (NO3 -) in human semen. With reference to the WHO method, the sperm survival rate, viability and the like were observed under an ultra-high magnification microscope. Results The concentration of NO in 15 male infertile patients was significantly lower than that of the normal control group (P <0.01). The abnormality of locomotor activity and the decrease of head swing amplitude were the main factors. There was no significant difference in sperm motility> 75% (P> 0.05). The concentration of NO in 65 cases was significantly higher than that of normal controls (a + b level <50% and survival rate <75% 0 0 1). Conclusion NO and sperm motility are closely related. High concentration significantly inhibited sperm motility and survival rate. Low concentrations of sperm survival and function has a protective effect, which causes male infertility research and treatment have very important clinical value.