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Six water-soluble polysaccharide-protein complexes coded as GM1, GM2, GM3, GM4, GM5 and GM6 wereisolated from the mycelium of Ganoderma tsugae by extracting with 0.2 mol/L phosphate buffer solution at 25, 40 and80℃, water at 120℃, 0.5 mol/L aqueous NaOH solution at 25 and 65℃, consecutively. Their chemical components wereanalyzed by using IR, GC, HPLC and ~(13)C-NMR, and some new results were obtained. The four samples GM1, GM2, GM3and GM4 are heteropolysaccharide-prote in complexes, in which, α- (1→3) linked D-glucose is the major monosaccharidewhile galactose, mannose and ribose are the secondary ones. GM5 and GM6 are β-(1→3)-D-glucan-protein complexes. Theprotein content increased from 32% to 69% with the progress of isolation. Weight-average molecu1ar mass M_w and theintrinsic viscosity [η] of the GM samples in 0.5 mol/L aqueous NaCl solution at 25℃ were measured systematically by laserlight scartering (LLS), size exclusion chromatography (SEC) combined with LLS, and viscometry. The M_w of GM1 to GM6are 35.5, 46.8, 58.9, 41.6, 3.3 and 22.0×10~4, respectively. The conformation and molecular mass of the two fractions of sample GM5 were characterized satisfactorily by SEC-LLS without further fractionation.
Six water-soluble polysaccharide-protein complexes coded GM1, GM2, GM3, GM4, GM5 and GM6 wereisolated from the mycelium of Ganoderma tsugae by extracting with 0.2 mol / L phosphate buffer solution at 25, 40 and 80 ° C., water at 120 ° C., 0.5 mol / L aqueous NaOH solution at 25 and 65 ° C, respectively. Their chemical components were analyzed by using IR, GC, HPLC and ~ (13) C-NMR, and some new results were obtained. The four samples GM1, GM2, GM3and GM4 are heteropolysaccharide-prote in complexes, in which α- (1 → 3) linked D-glucose is the major monosaccharides then galactose, mannose and ribose are the secondary ones. GM5 and GM6 are β- (1 → 3) -D- glucan-protein complexes. The protein content increased from 32% to 69% with the progress of isolation. Weight-average molecular mass M_w and theintrinsic viscosity [η] of the GM samples in 0.5 mol / by laserlight schering (LLS), size exclusion chromatography (SEC) combined with LLS, and The M_w of GM1 to GM6are 35.5, 46.8, 58.9, 41.6, 3.3 and 22.0 × 10 ~ 4, respectively. The conformation and molecular mass of the two fractions of sample GM5 were satisfactorily by SEC-LLS without further fractionation.