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目的:研究IL-13基因多态性位点rs20541和rs1800925单核苷酸多态性与儿童支气管哮喘发病的关联性。方法:应用聚合酶链反应(PCR)、等位基因特异性PCR(AS-PCR)和直接测序方法检测105例儿童哮喘患者和112名正常儿童的IL-13基因上rs20541和rs1800925单核苷酸多态性分布特点。结果:第4外显子中的+2044G→A(rs20541)哮喘组与正常对照组呈现明显差异(P<0.05)。而-1112C→T(rs1800925)位点两组间差异无统计学意义(P>0.05)。结论:+2044G→A(rs20541)与哮喘患儿的发病具有强烈相关性。-1112C→T无明显相关性。
OBJECTIVE: To study the association between SNP rs20541 and rs1800925 polymorphisms in IL-13 gene and the pathogenesis of bronchial asthma in children. Methods: The rs20541 and rs1800925 single nucleotide polymorphisms of IL-13 gene in 105 children with asthma and 112 normal children were detected by polymerase chain reaction (PCR), allele-specific PCR (AS-PCR) and direct sequencing Polymorphism distribution characteristics. Results: There was a significant difference between + 2044G → A (rs20541) asthma group and normal control group in exon 4 (P <0.05). There was no significant difference between the two groups at -1112C → T (rs1800925) (P> 0.05). Conclusion: + 2044G → A (rs20541) has a strong correlation with the onset of asthma in children. -1112C → T no significant correlation.