Analysis of Functional Group of Alkaline Phosphatase from DFRKN-1

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To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nematode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN-1 were analyzed in the paper. The results showed that Mg2+, Ba2+ and K+ under certain concentrations activated enzyme significantly and Zn2+ could inhibit enzyme activity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline phosphatase; sulfhydryl of cysteine was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phosphatase. To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nematode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN -1 were analyzed in the paper. The results showed that Mg2 +, Ba2 + and K + under certain concentrations activated enzyme significantly and Zn2 + could inhibit enzyme activity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline phosphatase; sulfhydryl of cysteine ​​was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phosphatase.
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肖南安 ,(XIAONan an 1940 - ) ,男 ,江西吉安人。 196 3年 7月于江西交通学院土木系道桥专业本科毕业。分配到内蒙古林业管理局基建处工作。先后任林业工程设计施工组组长、1980年 1
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