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To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nematode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN-1 were analyzed in the paper. The results showed that Mg2+, Ba2+ and K+ under certain concentrations activated enzyme significantly and Zn2+ could inhibit enzyme activity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline phosphatase; sulfhydryl of cysteine was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phosphatase.
To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nematode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN -1 were analyzed in the paper. The results showed that Mg2 +, Ba2 + and K + under certain concentrations activated enzyme significantly and Zn2 + could inhibit enzyme activity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline phosphatase; sulfhydryl of cysteine was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phosphatase.