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本实验采用自然和离体冻融(—22),冷冻蟾蜍眼球。将其分为在体和离体冻融。冷冻60分钟后,用0.2%甘油30℃复温,1—2分钟后复苏,速将其眼球经组织学常规处理。HE染色,olympus显微镜观察及图像分析冻融后蟾蜍眼球组织结构的变化。离体眼球组,显微镜下可见:角膜、视网膜、脉络膜、巩膜细胞体积增大,颗粒增多现象。纤维呈透明玻璃样变。在体冷冻复温组,眼球组织结构完整。各种细胞和纤维清楚可见,实验结果提示:蟾蜍眼球可以左—22℃条件下至少保存60分钟。0.1%甘油30℃复温。1—2分钟复苏。实验结果提示:眼球器官具有活力和功能。
In this experiment, natural and frozen in vitro (-22), frozen toad eye. Will be divided into in vivo and in vitro freeze-thaw. Frozen 60 minutes later, with 0.2% glycerol rewarming at 30 ℃, 1-2 minutes after the recovery, the eyeball will be routinely processed by histology. HE staining, olympus microscopy and image analysis toad eyeball ball structure changes after freezing and thawing. Isolated eye group, the microscope shows: cornea, retina, choroid, scleral cells increased in size, increased particle phenomenon. Fiber was transparent glass-like change. In vivo freezing rewarming group, eyeball structure is complete. Various cells and fibers clearly visible, the experimental results suggest that: toad eyeballs can be left at -22 ℃ for at least 60 minutes. 0.1% glycerol 30 ℃ rewarming. 1-2 minutes recovery. The experimental results suggest that the eyeball organ has vitality and function.