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目的:探讨ETV1蛋白在胃肠道间质瘤(gastrointestinal stromal tumor,GIST)组织中的表达及其临床意义。方法:收集山西医科大学第一医院2009-01-01-2012-06-30经外科切除的GIST组织标本84例,同时收集同院同期6例食管平滑肌瘤、3例胃神经鞘瘤、3例肠系膜神经纤维瘤、5例腹腔精原细胞瘤、5例胸腺瘤及10例正常胃肠道黏膜组织作为对照。应用免疫组织化学Polymer conjugate方法检测ETV1在GIST肿瘤组织和对照组组织中的表达,并与CD117和CD34标志进行比较观察。结果:ETV1、CD117和CD34在84例GIST组织中阳性表达率分别为96.4%(81/84)、100.0%(84/84)和76.2%(64/84),对照组真性平滑肌瘤、神经鞘瘤、纤维瘤病、精原细胞瘤、胸腺瘤及正常胃肠道黏膜组织中均未检测到ETV1蛋白的表达。CD117在精原细胞瘤和胸腺瘤中呈阳性表达,在平滑肌瘤、神经鞘瘤和神经纤维瘤中为阴性表达;CD34在对照组2例神经鞘瘤、1例神经纤维瘤中呈阳性表达,其余为阴性。ETV1在GIST中呈现不同程度的表达,表达强度与患者性别、年龄、肿瘤部位、病理形态及生物学行为无相关性,P>0.05。84例GIST中,ETV1表达与CD117表达基本呈一致性;ETV1与CD34的表达无相关性,χ2=0.972,P=0.324,r=0.107。ETV1标志GIST的敏感性(96.4%)与CD117相近(100.0%),明显高于CD34(76.2%);而特异性达100.0%,高于CD117(54.5%)和CD34(86.4%)。结论:ETV1是一种新的诊断GIST较特异标志,与CD117联合应用有望进一步提高GIST的诊断水平。
Objective: To investigate the expression of ETV1 protein in gastrointestinal stromal tumor (GIST) and its clinical significance. Methods: Totally 84 GIST tissue specimens were collected from the First Hospital of Shanxi Medical University during the period of 2009-01-01-2012-06-30. Six patients with esophageal leiomyoma, three gastric schwannoma, and three patients Mesenteric neurofibroma, 5 cases of peritoneal seminoma, 5 cases of thymoma and 10 cases of normal gastrointestinal mucosa as a control. The expression of ETV1 in GIST tumor tissues and control tissues was detected by immunohistochemistry Polymer conjugate method and compared with CD117 and CD34 markers. Results: The positive rates of ETV1, CD117 and CD34 in 84 cases of GIST tissues were 96.4% (81/84), 100.0% (84/84) and 76.2% (64/84), respectively. The positive leiomyomas, Schwannoma, fibromatosis, seminoma, thymoma and normal gastrointestinal mucosa were not detected ETV1 protein expression. CD117 was positive in seminoma and thymoma, negative in leiomyoma, schwannoma and neurofibromatosis. CD34 expression was positive in two schwannomas and one neurofibroma in the control group , The rest is negative. There was no correlation between the expression intensity and the gender, age, tumor location, pathology and biological behavior of ETV1 in GIST. There was no significant correlation between ETV1 expression and CD117 expression in GIST (P> 0.05) There was no correlation between ETV1 and CD34 expression, χ2 = 0.972, P = 0.324, r = 0.107. The ETV1 marker GIST sensitivity (96.4%) was similar to that of CD117 (100.0%), significantly higher than that of CD34 (76.2%); the specificity was 100.0%, higher than that of CD117 (54.5%) and CD34 (86.4%). Conclusion: ETV1 is a new specific marker for diagnosis of GIST. Combined with CD117, ETV1 is expected to further improve the diagnostic value of GIST.