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目的探索机体运动后糖原合成激素环境的变化以及补糖和补刺五加的干预效应,为补糖和刺五加促进运动后肌糖原恢复提供理论依据。方法 128只SD大鼠进行1周游泳训练,同时分别补充刺五加皂甙[500 mg/(kg.d)]、葡萄糖[6 g/(kg.d)]或生理盐水。随后进行糖原消耗运动,并于运动前后的不同时间点(运动前、运动后即刻、后4 h、后12 h)提取样本,测定血清胰岛素(insulin,INS)、胰高血糖素(glucagon,GL)水平和胰岛素受体-1(insu-lin receptor-1,IR-1)蛋白量。结果①补糖可以明显升高运动后大鼠的INS(U/mL)水平(24.50±2.46 vs19.15±1.56),同时补充刺五加有协同效应(24.59±3.88 vs19.15±1.56),而且这种效应一直持续至运动后12 h(29.67±3.26 vs 22.86±2.32)。②大鼠运动后GL(ng/L)水平明显升高(367.86±18.54 vs 221.76±22.28),运动后补充刺五加明显降低GL水平(328.62±20.10vs367.86±18.54)。③补糖对IR-1的蛋白量没有明显影响,而补充刺五加明显上调各时间点IR-1的蛋白量。结论补糖可以升高运动后INS水平,但是对IR-1蛋白含量的影响较小;补药对INS的影响较小,但升高了IR的蛋白含量。补药和补糖都降低了运动后血清GL的水平,两者同时补充的效果更好。
Objective To explore the change of glycogen synthesis hormones environment after exercise and the intervention effect of complementing and supplementing Acanthopanax senticosus, providing the theoretical basis for complementing Acanthopanax senticosus and promoting the recovery of muscle glycogen after exercise. Methods One hundred and eighty SD rats were subjected to one - week swimming training and were supplemented with 500 mg / (kg · d)], glucose [6 g / (kg · d)] or saline, respectively. The glycogen depletion was followed, and the samples were taken before and after exercise (before exercise, immediately after exercise, after 4 h, after 12 h), and the serum insulin, glucagon, GL) levels and the amount of insulin-1 receptor (IR-1) protein. Results (1) The level of INS (U / mL) was increased significantly (24.50 ± 2.46 vs 19.15 ± 1.56) in rats after exercise, while synergistic effect was observed in Acanthopanax senticosus (24.59 ± 3.88 vs 19.15 ± 1.56) And this effect continued until 12 h after exercise (29.67 ± 3.26 vs 22.86 ± 2.32). ② The level of GL (ng / L) was significantly increased after exercise in rats (367.86 ± 18.54 vs 221.76 ± 22.28). After exercise, acanthopanax senticosus significantly decreased the level of GL (328.62 ± 20.10vs367.86 ± 18.54). ③ sugar on the amount of protein IR-1 no significant impact, while the additional Acanthopanax significantly increased at each time point IR-1 protein. CONCLUSION: Intake can increase INS level after exercise, but has little effect on the content of IR-1 protein. Tonic has little effect on INS, but increases the protein content of IR. Tonic and sugar have reduced the level of serum GL after exercise, both at the same time complement the better.