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目的观察盐酸小檗碱(berberine hydrochloride,BBR)对溃疡性结肠炎模型小鼠结肠组织TNF-α、IL-1β和IL-10表达的影响,探讨其治疗UC的可能作用机制。方法 BALB/c小鼠随机分为模型对照组、BBR低、高剂量组、柳氮磺吡啶阳性对照组和空白对照组。采用右旋葡聚糖硫酸钠法复制UC小鼠模型后,每日灌胃给药1次,连续7 d。实验期间每天观察小鼠一般情况并评估疾病活动指数(DAI);末次给药后解剖观察并评估结肠大体形态损伤指数(CMDI);组织切片染色,光镜下观察结肠组织的病理学变化并评估组织损伤指数(TDI);ELISA酶联法检测结肠组织中细胞因子TNF-α、IL-1β和IL-10的含量。结果与模型对照组比较,BBR治疗组小鼠的结肠炎临床表现明显改善,其DAI、CMDI和TDI评分显著下降,小鼠结肠组织中TNF-α、IL-1β水平均明显降低,IL-10水平升高(P<0.05)。结论 BBR能有效治疗UC小鼠的结肠炎症,其机制可能与其抑制结肠组织中TNF-α和IL-1β、提高IL-10的表达有关。
Objective To investigate the effect of berberine hydrochloride (BBR) on the expression of TNF-α, IL-1β and IL-10 in colonic tissue of mice with ulcerative colitis and to explore its possible mechanism of action in treating UC. Methods BALB / c mice were randomly divided into model control group, BBR low and high dose group, sulfasalazine positive control group and blank control group. After the model of UC mice was duplicated by dextran sodium sulfate method, the mice were dosed once a day for 7 days. During the experiment, the daily condition of the mice was observed and the disease activity index (DAI) was observed. The gross morphology damage index (CMDI) of the colon was observed and evaluated after the last administration. The pathological changes of colon tissues were observed under light microscopy and evaluated Tissue damage index (TDI) was measured. The contents of TNF-α, IL-1β and IL-10 in colon tissues were detected by ELISA. Results Compared with the model control group, the clinical manifestations of colitis in BBR treatment group were significantly improved, the scores of DAI, CMDI and TDI were significantly decreased, while the levels of TNF-α and IL-1β in colonic tissue of mice were significantly decreased Elevated levels (P <0.05). Conclusion BBR can effectively treat colonic inflammation in UC mice, and its mechanism may be related to the inhibition of TNF-α and IL-1β in colonic tissue and the increase of IL-10 expression.