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采用毒性标准实验方法研究了Cd2+与S-异丙甲草胺单独及联合作用对斜生栅藻急性毒性、总可溶性蛋白含量、超氧化物歧化酶(SOD)活性和细胞膜通透性的影响.结果表明Cd2+与S-异丙甲草胺单独作用时EC50均随时间的延长而减小,且S-异丙甲草胺的急性毒性大于Cd2+;Cd2+、S-异丙甲草胺的EC50-24h分别为0.27 mg·L-1、0.24 mg·L-1,EC50-96h分别为0.16mg·L-1、0.13 mg·L-1.Cd2+与S-异丙甲草胺联合作用时低浓度表现为协同作用,高浓度表现为拮抗作用.暴露96 h后,Cd2+与S-异丙甲草胺单独及联合作用下,随有毒物质浓度升高,斜生栅藻总可溶性蛋白含量降低,SOD酶活性先激活后抑制,细胞膜通透性逐渐增大.
The effects of Cd2 + and S-metolachlor alone and in combination on the acute toxicity, total soluble protein content, superoxide dismutase (SOD) activity and cell membrane permeability were studied by toxicity standard method. The results showed that the EC50 of Cd2 + and S-metolachlor decreased with time, and the acute toxicity of S-metolachlor was greater than that of Cd2 +; the EC50- 24h, respectively, 0.27 mg · L-1, 0.24 mg · L-1, EC50-96h respectively 0.16mg · L-1, 0.13 mg · L-1.Cd2 + S-metolachlor combined with low concentrations Showed synergy and antagonism at high concentration.After 96 h exposure, Cd2 + and S-metolachlor alone and in combination, with the concentration of toxic substances increased, the total soluble protein content of Scutella: Enzyme activity first activated after inhibition, cell membrane permeability gradually increased.