论文部分内容阅读
本实验研究了脑源性神经营养因子(brain derived neurotrophic factor,BDNF),神经生长因子(nerve growth factor,NGF)和神经营养因子-3(neurotrophin-3,NT-3)的mRNA和蛋白在猫左侧腰第六背根节(dorsal root ganglion,DRG)共表达的不同方式,并探讨了共表达的机制,为阐明神经营养因子的表达与脊髓可塑性的关系提供依据。本实验所使用的为未接受任何处理的健康猫。猫的左侧腰第六DRG被取出,行免疫组织化学染色与原位杂交方法结合的双重标记,确定是否有BDNF,NGF和NT-3的蛋白与mRNA共表达。实验结果显示BDNF,NGF和NT-3的蛋白与mRNA在猫DRG均有表达,但这三种神经营养因子mRNA和蛋白共表达的方式是不同的。免疫组化结果显示:BDNF阳性产物主要分布于细胞质和细胞核,细胞核的染色颜色较细胞质浅;NGF阳性产物主要分布于细胞核;NT-3阳性产物主要分布于细胞质。原位杂交结果显示:BDNF和NGF阳性信号主要分布于细胞质;NT-3阳性信号在细胞质和细胞核都有分布。由此可见,BDNF,NGF和NT-3的mRNA和蛋白在猫左侧腰第六DRG有不同的共表达方式,提示它们可能存在与脊髓可塑性有关的自分泌和/或旁分泌机制。
This study investigated the effects of brain derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3) Different ways of co-expression of dorsal root ganglion (DRG) in the left lumbar and discuss the mechanism of co-expression in order to provide basis for elucidating the relationship between the expression of neurotrophic factor and spinal cord plasticity. This experiment is used to not receive any treatment of healthy cats. The sixth DRG on the left lumbar of the cat was removed and double-labeled by immunohistochemical staining combined with in situ hybridization to determine whether proteins and mRNAs of BDNF, NGF and NT-3 were co-expressed. The results showed that the protein and mRNA of BDNF, NGF and NT-3 were all expressed in the DRG of cats. However, the co-expression of mRNA and protein of these three neurotrophic factors was different. The results of immunohistochemistry showed that the positive products of BDNF mainly distributed in the cytoplasm and nucleus, the staining of nucleus was lighter than the cytoplasm; the positive products of NGF were mainly distributed in the nucleus; the positive products of NT-3 were mainly distributed in the cytoplasm. In situ hybridization results showed that positive signals of BDNF and NGF mainly distributed in the cytoplasm; NT-3 positive signals were distributed in cytoplasm and nucleus. Thus, mRNA and protein expressions of BDNF, NGF and NT-3 were differently co-expressed in the sixth DRG on the left side of the cat, suggesting that there may be autocrine and / or paracrine mechanisms associated with spinal cord plasticity.