提高微核试验质量的关键是找到一种能避免细胞损害的溶液。作者以骨髓细胞为材料,分别用1%柠檬酸三钠低张液,生理盐水,Ringer 氏盐水,市售等张液(Isoton)及甲醛盐水(0.85%NaCl 在4%甲醛的水溶液中)处理标术,研究这些溶液对微核试验的影响。结果表明,甲醛盐水明显优于其它介质,使骨髓细胞形态保存完好,显著提高涂片质量和可重复性,试验程序易于掌握,控制,以及缩短微核染色时间。另外,用环磷酰胺诱导微核试 The key to improving the quality of a micronucleus test is to find a solution that avoids cell damage. The myeloid cells were treated with 1% trisodium citrate hypotonic solution, physiological saline, Ringer’s saline solution, commercially available isotonic solution of Isoton and formalin saline (0.85% NaCl in 4% formaldehyde in water) The standard, to study the impact of these solutions on micronucleus test. The results showed that formaldehyde saline was significantly better than other media, the morphology of bone marrow cells intact, significantly improve the quality and repeatability of the smear, test procedures easy to grasp, control, and shorten the time of micronuclei. In addition, micronucleus test was induced with cyclophosphamide