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目的参考多篇文献建立用于体外药物抗ＨＩＶ研究的ＭＴＴ试验方法。方法其过程可简述为先用９６孔微量板做抗病毒实验，４天后，加ＭＴＴ（０．７ｍｇ／ｍｌ）２０μｌ／孔，置３７℃ＣＯ２箱中４ｈ，移去１５０μｌ／孔培养液，再加１００μｌ／孔的ＤＭＳＯ过１０分钟，即可测定ＯＤ５４０值，计算药物对ＨＩＶ感染细胞的保护率。结果ＨＬ的ＣＣ５０＝０．３３５４ｍｇ／ｍｌ，ＥＣ５０＝０．０８６４ｍｇ／ｍｌ，ＭＴＴ法较ＣＰＥ法更为敏感。结论ＭＴＴ法能够以光密度值快速、客观、准确地得到试验结果，提高实验的质量和效率
OBJECTIVE To establish a MTT assay for the anti-HIV study of in vitro drugs with reference to several articles. Methods The process can be described briefly as an antiviral experiment with a 96-well microtiter plate. After 4 days, add MTT (0.7mg / ml) 20μl / well and incubate at 37 ℃ for 4h. Remove 150μl / well medium, Add 100 μl / well of DMSO over 10 minutes to determine the OD540 value and calculate the drug’s protection against HIV-infected cells. Results HL50 CC50 = 0.3354mg / ml, EC50 = 0.0864mg / ml, MTT method is more sensitive than the CPE method. Conclusion The MTT method can quickly, objectively and accurately obtain the experimental results with the optical density value, and improve the quality and efficiency of the experiment