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以1对近等基因系(NILs)构建的分离群体(NT4092/NT411)F1为材料,采用自然发病鉴定方法,以行发病率为表型值,在3种环境下对各株系进行粗缩病抗病性鉴定。应用完备区间作图法(ICIM)对玉米粗缩病抗性QTL进行分析,分离群体(NT4092/NT411)F1在两地三点检测到3个QTL,均位于第8染色体上,LOD值分别为24.03、10.29和17.02,分别解释了41.37%、20.51%和36.04%的表型变异,是能够稳定表达的主效QTL。泰安5月份和济宁5月份环境下检测到的主效QTL均位于phi121和UMC1817标记区间之内,遗传距离为15.47 cM,标记区间物理距离为92Mb;泰安6月份环境下检测到的主效QTL位于M6和M24标记区间之内,遗传距离为0.86 cM,标记区间物理距离为17.9 Kb。通过对4个分离群体辅助选择的可行性分析表明,这些主效QTL与玉米粗缩病抗性间呈极显著相关,其标记在CL313、1145、沈137等系谱来源相同或相近的自交系中可应用于抗玉米粗缩病育种的辅助选择。
A population of isolates (NT4092 / NT411) constructed from one pair of near-isogenic lines (NILs) was used as material. The natural disease identification method was used to determine the incidence of disease as the phenotypic value. Disease resistance identification. QTLs for resistance to maize rough shrub were analyzed by using integrated interval mapping (ICIM). Three QTLs were detected on F1 population from two populations at three loci (NT4092 / NT411), all locating on chromosome 8 with LOD values of 24.03, 10.29 and 17.02, which explained the phenotypic variation of 41.37%, 20.51% and 36.04%, respectively, which were the major QTLs for stable expression. The major QTLs detected in May and Jining in May were located within the phi121 and UMC1817 marker intervals with a genetic distance of 15.47 cM and a marker range of 92Mb. The major QTLs detected in Tai’an in June were located in Within the M6 and M24 marker intervals, the genetic distance was 0.86 cM and the marker interval physical distance was 17.9 Kb. Through the feasibility analysis of the auxiliary selection of the four isolation groups, it showed that the major QTLs were significantly correlated with the resistance to maize disease. The markers QTLs were significantly different between inbred lines with the same or similar pedigree (CL313, 1145, Shen 137) Which can be applied to the auxiliary selection of anti-maize rough breeding.