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目的 :研究衰老相关 β 半乳糖苷酶 (SA β Gal)组化染色在不同类型组织切片中的应用。方法 :比较血涂片、冰冻切片及石蜡切片在不同条件下SA β Gal的显色效果。结果与结论 :冰冻切片结果良好 ;石蜡切片经抗原修复后 ,结果不及未经抗原修复者 ;血涂片以 40g/L多聚甲醛固定 45min者效果最佳。SA β Gal可用于不同类型的组织标本。
Objective: To study the application of senescence-associated β-galactosidase (SA β Gal) histochemical staining in different types of tissue sections. Methods: The color development of SA β Gal under different conditions was compared between blood smears, frozen sections and paraffin sections. RESULTS AND CONCLUSION: The results of the frozen section were good. After the paraffin section was repaired by antigen, the results were less than those without antigen repair. The blood smears were best fixed with 40g/L paraformaldehyde for 45min. SA β Gal can be used for different types of tissue specimens.