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采用Echandi方法,测定了从国内不同植物上分离,经鉴定的3个种和亚种的软腐欧氏杆菌(Erwiniacarotovora var.carotovora Dye,E.carotovora var.otroseptica Dye,E.chrysanthemi Burkholder.etal.)的224个菌株产生细菌素的能力。指示菌为代表3个不同种的菌株。测定结果表明:224个试验菌株中能产细菌素的有97个,占总数的43.3％。经测定产生细菌素的专化性有所不同,约各有1/3菌株分别对3种、2种、1种指示菌有抑制作用。有些广谱的细菌素甚至对其它属的植物病原细菌也有抑制作用。少数种内专化的产细菌素菌株可望用于软腐欧氏杆菌种鉴定的辅助手段。电镜观察初步表明,这些细菌素可能是分子大小不同的两种类型的细菌素:小分子细菌素在电镜下不可见,大分子细菌素具有短杆状结构,颇似无头壳的噬菌体的尾部。
The Echandi method was used to determine the three species and subspecies of Erwinia carotovora Dye, E. carotovora var. Otroseptica Dye, E. chrysanthemi Burkholder. Et al., Isolated and identified from different plants in China. ) Of 224 strains of bacteriocin production capacity. Indicator bacteria represent three different strains. The results showed that 97 of the 224 test strains could produce bacteriocin, accounting for 43.3% of the total. After the determination of the specificity of producing bacteriocins vary, about 1/3 of the strains were 3 kinds, 2 kinds, 1 kinds of indicator bacteria have inhibitory effect. Some broad-spectrum bacteriocins even have an inhibitory effect on phytopathogenic bacteria of other genera. A few kinds of internalized bacteriocin producing strains are expected to be used as auxiliary means for the identification of soft rots. Electron microscopy initially showed that these bacteriocins may be two types of bacteriocins of different molecular sizes: the small molecule bacteriocin is not visible under electron microscopy, the large bacteriocin has a short rod-like structure, resembling the headless phage tail .