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采用焦锑酸钾沉淀法研究了温敏雄性不育水稻(Oryza sativa L.)培矮64S在高温引起雄性不育与正常可育花药发育过种中Ca2+的分布变化。结果表明,当培矮64S生长在较高温度条件下引起雄性不育,与可育花药相比,不育花粉母细胞中有较多的液泡、较多的Ca2+沉积和较少的线粒体,并且有较多的Ca2+沉积在不育花药的中间层、表皮层和绒毡层中。到四分体与单细胞花粉时期,不育花药的木质部细胞的次生加厚壁上有较多的Ca2+沉淀,连接组织中的Ca2+沉淀也大大增加,所有不育花粉外壁较厚而发育都不正常。在单核细胞早期,不育花粉的四分体细胞中有较明显的大液泡出现。不育花药中的Ca2+在花药发育的各时期均比可育花药要多。这些结果说明在高温生长条件下,花粉母细胞发育的异常、花药中Ca2+沉积的增加、绒毡层与花粉外壁发育的异常可能与培矮64S花粉败育相关。
The change of Ca2 + distribution in male sterile and normal fertile anther of over temperature-sensitive male sterile rice (Pei’ai 64S) was studied by using potassium antimonite precipitation method. The results showed that when Peiai 64S was grown at higher temperature to cause male sterility, more vacuoles, more Ca2 + deposits and less mitochondria were found in sterile pollen mother cells compared with fertile anthers, and More Ca2 + was deposited in the middle layers, epidermis and tapetum of sterile anthers. To tetrad and single-cell pollen stage, there were more Ca2 + precipitates on the secondary thickened walls of xenoblasts of sterile anthers, and the Ca2 + precipitation in connective tissues was also greatly increased. All sterile pollen outer walls were thicker and developed unusual. In the monocyte early, sterile pollen tetrad cells have more obvious large vacuole appears. Ca2 + in sterile anthers is more than that in fertile anthers at each stage of anther development. These results indicate that the abnormal development of pollen mother cells, the increase of Ca2 + deposition in anther, the abnormal development of tapetum and pollen wall may be related to the pollen abortion of Peiai 64S at high temperature.