论文部分内容阅读
目的分析蒲公英的遗传多样性。方法采用ISSR分子标记对21个居群蒲公英遗传多样性进行分析,用软件Popgen32分析Nei’s基因多样性指数(H)和Shannon信息指数(I),采用NTSYS 2.10e软件进行聚类分析和主坐标分析。结果 16条ISSR引物共扩增出166条条带,其中多态性条带152条,平均多态性百分率为90.4%,平均H为0.286 5,平均I为0.437 0;遗传距离和遗传一致度分别为0.156 2～0.590 2和0.554 2～0.855 4;UPMGA法进行聚类分析显示,聚类结果与地理来源有较强的一致性,可以看出来源于同一地区的蒲公英种质聚在一起,呈现出一定的地域性分布规律。结论采用ISSR分析蒲公英遗传多样性,蒲公英表现出丰富的多态性,表明可以用ISSR标记技术对蒲公英进行分子水平的遗传多样性分析。
Objective To analyze the genetic diversity of dandelion. Methods ISSR markers were used to analyze the genetic diversity of 21 populations of Dandelion. Nei’s gene diversity index (Shannon index) and Shannon’s information index (I) were analyzed by Popgen32 software. NTSYS 2.10e software was used for cluster analysis and principal coordinate analysis . Results Sixteen ISSR primers amplified 166 bands, of which 152 bands were polymorphic. The average percentage of polymorphic bands was 90.4%. The average H was 0.286 5 and the average I was 0.437 0. The genetic distance and genetic identity Respectively from 0.156 2 to 0.590 2 and from 0.554 2 to 0.855 4. Cluster analysis by UPMGA showed that the clustering results were strongly consistent with the geographical origin. It can be seen that the dandelion germplasm collected from the same area together, Showing a certain geographical distribution. Conclusion ISSR analysis of genetic diversity of dandelion, dandelion showed rich polymorphism, indicating that genetic diversity can be molecular level analysis of dandelion ISSR markers.