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用免疫组织化学方法分别检测了乙型肝炎病毒携带者(ASC)、慢性乙型肝炎(CH)和肝癌(HCC)患者肝组织内增殖细胞核抗原(PCHA),抑癌基因p16和凋亡基因FAS的表达情况。结果,PCNA阳性率ASC组为500%,CH组为676%,均明显低于HCC组的967%(P<0.01),而FAS阳性率ASC组为813%,CH组为690%,均高于HCC组的333%(P<0.01)。p16阳性率ASC组为375%、CH组为225%,均高于HCC组的33%(P<0.01,P<0.05)。乙肝病毒核心抗原(HBcAg)阳性组FAS阳性率为889%,高于HBcAg阴性组的633%(P<001)。PCNA阳性率HBcAg阳性组为852%,也高于HBcAg阴性组的550%(P<0.05)。结果提示,HBV感染后引起的癌基因、抑癌基因、凋亡基因表达的变化及其相互作用可能在肝细胞癌形成过程中具有重要作用。
Immunohistochemistry was used to detect the expression of proliferating cell nuclear antigen (PCHA), tumor suppressor gene p16 and apoptotic gene FAS in liver tissues of patients with chronic hepatitis B (CH) and hepatocellular carcinoma (HCC) The expression of the situation. The positive rate of PCNA was 500% in ASC group and 676% in CH group, which were significantly lower than that in HCC group (P <0.01). The positive rate of PCNA was 813% in ASC group and 690% in CH group 333% of HCC patients (P <0.01). The positive rate of p16 was 375% in ASC group and 225% in CH group, all higher than that in HCC group (P <0.01, P <0.05). The positive rate of FAS in HBsAg positive group was 889%, which was higher than that in HBcAg negative group (633%, P <001). The positive rate of PCNA in HBcAg positive group was 852%, which was also higher than that in HBcAg negative group (P <0.05). The results suggest that the changes and interactions of oncogenes, tumor suppressor genes and apoptotic genes induced by HBV infection may play an important role in the development of hepatocellular carcinoma.