最近,美国NCI的Pastan等人研究发现一种人癌细胞系KB在获对某些抗癌药物耐受性的同时,伴有特定基因的扩增。美国MIT的Roninson于1983年建立了一种直接在琼脂糖凝胶内快速检测基因组内扩增基因的方法。此法只需经一简单处理过程,即能使以数十或数百个拷贝存在于基因组内的扩增基因在模糊的背景上形成清晰的杂交条带而被检出。 本文应用Roninson建立的方法研究食管癌发生过程中的基因扩增情况。以正常胎儿食管基因组DNA为正常对照;对4例食管癌及其癌旁组织,2例食管癌组织,1例以MNNG诱发恶性转化的食管癌成 Recently, the United States NCI’s Pastan et al. Found that a human cancer cell line KB is resistant to certain anticancer drugs accompanied by amplification of specific genes. In 1983, Roninson at MIT in the United States established a method for rapidly detecting genomic amplified genes directly in agarose gels. This method requires only a simple process to detect amplified amplicons in the background of a blurry background with tens or hundreds of copies of the amplified gene present in the genome. In this paper, Roninson established the method to study the gene amplification during esophageal carcinogenesis. Normal fetal esophageal genomic DNA as a normal control; 4 cases of esophageal cancer and its adjacent tissues, 2 cases of esophageal cancer, 1 case of MNNG induced malignant transformation of esophageal cancer into